Figure 3.

Novel missense variants in dOgdh are lethal in a Drosophila model. A. A schematic of the expression of Gal4 from dOgdh-T2A-Gal4 allele and Gal4/UAS-mediated expression of wild-type or mutant dOgdh cDNA. B. A table displaying the missense variants in human OGDH and their homologous variants in Drosophila Ogdh (dOgdh). C. The lethality caused by dOgdh-T2A-Gal4 null allele was rescued by dOgdh wild-type cDNA expression. Expression of dOgdh carrying p.(Pro193Leu) or p.(Ser301Tyr) failed to rescue the lethality of dOgdh null mutant. cDNA, complementary DNA; gRNA, guide RNA; OGDH, oxoglutarate dehydrogenase; UAS, upstream activating sequence.