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. 2022 Jul 12;39(2):177–193. doi: 10.1007/s12264-022-00910-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Expression of TMEM63A in human nerve and neuroma. A Representative images of a human nerve and neuroma. Left, image from the surgical procedure for dissecting the neuroma from an amputee; middle, the dissected neuroma; right, part of a human tibial nerve acutely isolated from a patient subjected to amputation due to a traffic accident (scale bar, 20 mm). B qRT-PCR results for Tmem63a expression in human tibial nerve and neuroma samples (n = 2–3, **P <0.01, unpaired Student’s t-test). C Immunostaining for TMEM63A with anti-TMEM63A primary antibody in the human nerve and neuroma tissue (scale bar, 200 μm). D Relative fluorescence intensity of TMEM63A in (C) (n = 24–25; ***P <0.001, unpaired Student’s t-test).