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. 2022 Jul 12;39(2):177–193. doi: 10.1007/s12264-022-00910-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Generation of Tmem63a global knockout mice. A Schematic of the strategy for generating Tmem63a global knockout mice. B Genotyping of Tmem63a^–/– mice showing homozygous (Tmem63a^–/–), heterozygous (Tmem63a^+/–), and WT (Tmem63a^+/+) mice with the wild-type band at 600 bp and the knockout band at 504 bp. C RT-PCR results for the knockout efficiency of Tmem63a in global knockout mice, three biological repeats per genotype. Tmem63a band at 163 bp and Gapdh band at 100 bp. D Representative images of ISH combined with IHC with pan neuronal marker β-tubulin III (green) to determine efficient knockout of Tmem63a (red) in DRGs (scale bars, 200 μm).