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. 2023 Feb 7;9:21. doi: 10.1038/s41531-023-00445-9

Fig. 5. Detection of LRRK2 & Rab10 in rat organ lysates and in urinary EVs after solvent and LRRK2 kinase inhibitor treatment.

Fig. 5

a Western blot detection of total LRRK2, pS935-LRRK2, total Rab10, pT73-Rab10 and TSG101 in kidney, brain and lung lysates of rats treated with PF360 or solvent (7.5 mg/kg, 6 h). Note that the blots in kidney yield a non-specific band at a higher molecular weight. b Detection of total LRRK2 in normal rat uEVs, showing very low LRRK2 levels. c Western blot detection of total total Rab10, pT73-Rab10 and TSG101 in rat uEVs, both pre-treatment and after administration of PF360 or solvent (7.5 mg/kg, 6 h) (triplicate measures per sample). d quantification of (c). The values of phosphorylation ‘rate’ are indicative of the proportion of protein that is phosphorylated at the given site, see materials and methods. Statistical differences were tested using a two-tailed Mann–Whitney test. Error bars represent standard error of the mean (s.e.m.). *P < 0.05 (N = 8–9).