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. 2023 Feb 7;14(2):90. doi: 10.1038/s41419-022-05547-5

Fig. 4. PDHB-AS competitively binds to miR-582-5p to enhance DKK1 expression.

Fig. 4

A FISH assay detected the distribution of PDHB-AS in CC cells. B miR-582-5p that might bind with PDHB-AS and DDK1 was predicted via starBase and DIANA. C RNA pull down assays detected the enrichment of PDHB-AS and DKK1 in Bio-miR-582-5p-WT/MUT. D The binding sites of miR-582-5p on DKK1 and PDHB-AS were predicted severally by DIANA and starBase. E RT-qPCR detected miR-582-5p expression in HeLa and SiHa cells after miR-582-5p overexpression. F The luciferase activity of indicated groups was measured for further confirmation of the affinity between miR-582-5p and DKK1/PDHB-AS. **P < 0.01.