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. 2023 Feb 7;14(2):90. doi: 10.1038/s41419-022-05547-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A, B The mRNA and protein levels of Wnt7b were measured respectively by RT-qPCR and western blot after miR-582-5p overexpression. C PABPC1 and RBMX were ascertained to bind to PDHB-AS by RNA pull down assays and mass spectrometry, followed by western blot to detect PABPC1 and RBMX protein levels precipitated in PDHB-AS sense/antisense, with input as a positive control. D, E In RIP assay, the relative enrichment of PDHB-AS and Wnt7b in anti-PABPC1 and anti-RBMX groups was detected via RT-qPCR, with IgG as a negative control. F, G The mRNA and protein levels of Wnt7b were examined respectively by RT-qPCR and western blot after knockdown of PABPC1. H In RIP assay, the relative enrichment of PDHB-AS or Wnt7b precipitated in anti-PABPC1 was measured in cells transfected with pcDNA3.1 or pcDNA3.1-PDHB-AS, respectively. I, J RT-qPCR and western blot analyses of Wnt7b levels were performed in HeLa and SiHa cells transfected with pcDNA3.1, pcDNA3.1-PDHB-AS, pcDNA3.1-PDHB-AS + si-NC, or pcDNA3.1-PDHB-AS + si-PABPC1. **P < 0.01, n.s.: no significance.