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. 2023 Feb 7;14:666. doi: 10.1038/s41467-023-36349-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative cryo-electron micrograph of A. tumefaciens T-pilus, from 8,363 images collected. Scale bar, 50 nm. b Side and top view of the T-pilus cryo-EM density map at a resolution of 3.5 Å. The front half of the filament has been removed in the side view, and we are looking at the lumen. c While there is a 1:1 stoichiometry of lipids to pilins in the filament, a pilin (red) makes contact with four lipids (green). d Atomic model of the T-pilus in ribbon representation docked within the transparent cryo-EM density map. A single subunit model is shown in red. An inset on the right shows front and back views of the VirB2 subunit.