Skip to main content
. 2023 Jan 25;14:1105244. doi: 10.3389/fimmu.2023.1105244

Figure 1.

Figure 1

3D co-culture of PDTOs and DCs: Setup, morphology, and viability. (A) Schematic representation of the co-culture system and the three different conditions used in this study: DCs cultured alone, DCs and PDTOs co-cultured, and PDTOs cultured alone. (B) Closeup and morphology of iDCs and mDCs. The PDTOs are derived from CRC liver metastasis of two different patients, PDTO cystic as the name suggests presents a cystic morphology, whereas PDTO dense has a compact morphology. (C) Quantification of mDCs and iDCs viability when cultured alone in 3D in the collagen matrix after 48h, based on NucBlue™ Live and NucGreen™ Dead stainings, in two different experiments/donors. (D) The viability of mDCs, iDCs, and PDTOs was evaluated, after 48h of co-culture, during live imaging with NucBlue™ Live reagent (staining the nuclei of all cells) and NucGreen™ Dead reagent (staining only dead cells). The large majority of DCs seem to be viable alone, and in co-culture. iDCs - immature MoDCs, mDCs - mature MoDCs, PDTOs - patient-derived tumor organoids.