Figure 2. Pan-protein staining reveals P granules as protein-dense perinuclear structures.

(A) Pan-protein (NHS ester) and anti-GFP staining of animals expressing gfp::deps-1. Pan-protein staining reveals a number of features including P granules. GFP-DEPS-1 condensates (green) are localised to the P granule (gray). White arrows highlight P granules that are enlarged in (B). Scale bar = 10 µm. (B) Zoomed images of P granules highlighted by arrowheads in (A). DEPS-1 condensates appear as small protein clusters that are localised to P granules. Scale bar = 2 µm. (C) Number of granules observed per nucleus in a single optical slice. Granule is defined as perinuclear density observed either via GFP-DEPS-1 staining (green) or pan-protein staining (NHS ester; gray). 12 nuclei from four independent experiments were counted. Counts obtained from the same nucleus have the same colour.

Source data are available for this figure.