Figure 3. Mutator foci are juxtaposed to PRG-1 condensates.

(A) Pan-protein (NHS ester), anti–PRG-1, and anti-GFP staining of animals expressing GFP-tagged MUT-16. PRG-1 (red) and MUT-16 (green) colocalise to P granules (gray). MUT-16 and PRG-1 occupy distinct areas, whereby MUT-16 is frequently observed on the edge of the P granule space. White arrowheads in merged image highlight granules that are enlarged in (B). Scale bar = 10 µm. (B) Zoomed image of granules marked by arrowheads in (A). PRG-1 (red) exists as small cluster of proteins within the P granule. White line outlines the P granule boundary based on pan-protein staining (gray). MUT-16 (green) appears as single clusters that are either inside the P granule (granules 1, 3, and 4) or outside the P granule (granules 1 and 2). Scale bar = 2 µm. For each granule, the intensity of the staining was measured along the white arrow (inset of the plots) and normalized to the intensity of the entire granule to show the distribution of MUT-16 (green) and PRG-1 (red) relative to germ granule (gray).

Source data are available for this figure.