Figure 4. ZNFX-1 and PRG-1 condensates are subdomains within the same germ granules.

(A) Pan-protein (NHS ester), anti–PRG-1, and anti-GFP staining of animals expressing GFP-tagged ZNFX-1. PRG-1 (red) and ZNFX-1 (green) colocalise to P granules (gray). ZNFX-1 and PRG-1 occupy distinct, and overlapping areas within the P granule space. White arrowheads in merged image highlight granules that are enlarged in (B). Scale bar = 10 µm. (B) Zoomed image of granules marked by arrowheads in (A). Both PRG-1 (red) and ZNFX-1 (green) exist as small clusters of proteins within the P granule. The white line outlines the P granule boundary based on pan-protein staining (gray). ZNFX-1 is concentrated in areas closer to the cytoplasmic edge of the P granule than PRG-1 (granules 1, 3, and 4). Scale bar = 2 µm. For each granule, the intensity of the staining was measured along the white arrow (inset of the plots) and normalized to the intensity of the entire granule to show the distribution of ZNFX-1 (green) and PRG-1 (red) relative to germ granule (gray).

Source data are available for this figure.