Figure 5. P granules are malformed in animals defective in small RNA pathways.

(A) Pan-protein (NHS ester) and anti-GFP staining of animals expressing gfp::deps-1. Scale bar = 10 µm. (B) Pan-protein (NHS ester) and anti–PRG-1 staining of deps-1(bn124) mutant animals. deps-1 mutation leads to a reduction in the size of germ granules. Arrowheads highlight a germ granule that is mislocalised. Scale bar = 10 µm. The intensity of the staining was measured along the white arrow (inset of the plot; scale bar = 2 µm) and normalized to the intensity of the entire granule to show the distribution of PRG-1 relative to the germ granule. (C) Pan-protein (NHS ester), anti-DEPS-1, and anti–PRG-1 staining of mut-16 (pk710) mutant animals. mut-16 mutation leads to a reduction in the size of germ granules. Scale bar = 10 µm. (D) P granule size in WT animals, deps-1 (bn124), and mut-16 (pk710) mutants. P granule size was calculated by measuring the maximum length of the P granule perpendicular to the nuclear membrane and normalizing it to the diameter of the nucleus. P granules are smaller in both mutants compared with WT animals. *P-value < 0.001 and **P-value < 0.0001.

Source data are available for this figure.