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. 2023 Jan 25;13:1089286. doi: 10.3389/fimmu.2022.1089286

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Copyright © 2023 Zong, Kamoi, Kurozumi-Karube, Zhang, Yang and Ohno-Matsui

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Levels of inflammatory cytokines were measured in the culture supernatants of HRPEpiCs and HRPEpiCs co-cultured for 48 h with MT2, TL-Om1, or Jurkat cells with/without 0.5 mg/mL aflibercept. In co-culture of HRPEpiCs with MT2 cells, aflibercept did not significantly affect the levels of IL-6, IL-8, and IFN-γ. In co-culture of HRPEpiCs with TL-Om1 cells, production of IL-6 increased significantly following aflibercept addition, but levels of IL-8 and IFN-γ did not change significantly. Data are taken from three independent biological experiments and presented as the mean ± SEM (units: pg/μL) (*P < 0.05; n.s., not significant).