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. 2023 Jan 25;14:1090026. doi: 10.3389/fpls.2023.1090026

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Copyright © 2023 Suanno, Tonoli, Fornari, Savoca, Aloisi, Parrotta, Faleri, Cai, Coveney, Boocock, Verderio and Del Duca

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EVs from germinated kiwi pollen (A), EVs from kiwi pollen hydrated in a humid chamber (B), and EVs from kiwi pollen hydrated in PBS (C), dyed with FM4-64™ to enhance the presence of double-layered vesicles. (D, E) show FM4-64™ staining on the pollen tube apical region at different magnifications, as a positive control.