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. 2023 Jan 25;14:1090026. doi: 10.3389/fpls.2023.1090026

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Copyright © 2023 Suanno, Tonoli, Fornari, Savoca, Aloisi, Parrotta, Faleri, Cai, Coveney, Boocock, Verderio and Del Duca

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analysis of pollen total lysate (TL) and nanovesicles (EVs) fractions from germinated kiwi pollen (GKP). Samples were probed for the presence of molecular markers of different cell compartments with the following rabbit polyclonal antibodies: 1:2000 dilution of anti-clathrin heavy chain, 1:5000 dilution of anti-H+ATPase, 1:500 dilution of anti-COXII, 1:5000 dilution of anti-UGPase, 1:1000 dilution of anti-ARF1. The membranes were developed with ECL and read in chemiluminescence. On the left are reported the molecular weights of the pre-stained ladder, while on the bottom are reported the protein amounts loaded per lane.