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. 2023 Jan 25;14:1090026. doi: 10.3389/fpls.2023.1090026

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Copyright © 2023 Suanno, Tonoli, Fornari, Savoca, Aloisi, Parrotta, Faleri, Cai, Coveney, Boocock, Verderio and Del Duca

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of ALIX-homologs in kiwi pollen tube. (A–C): indirect immunofluorescence labeling with FITC-conjugated secondary antibodies, probed for ALIX-homologs, at different magnifications.Tthe fluorescent labeling of the target proteins is pictured in green. In A, the autofluorescence of the pollen wall is visible (*). (D–F): immunogold labeling of ALIX-homologs in transverse sections of kiwi pollen tube. Images were taken at the level of apex and subapex. Some of the proteins appear associated with subcellular structures compatible with vesicles (arrows), near the plasma membrane. PTW, pollen tube wall; PTM, pollen tube plasma membrane.