Fig. 3. Effect of miR-1246 overexpression on Th17 subsets in peripheral CD4⁺ T cells. (A) Flow cytometry assay of the purity of peripheral CD4⁺ T cells sorted by magnetic beads, including the miR-1246 lentiviral vector transduced cells (miR-1246 group), the negative control vector transduced cells (negative control group), and the blank control group. (B) miR-1246 expression mediated by transduction. (C) ROR-γt and IL-17 mRNA levels of transduced cells. (D) Proportion of Th17 cells in the transduced cells. (E) Concentration of cytokines in the supernatant of the transduced cell culture. miR-1246: microRNA-1246, ROR-γt: retineic-acid-receptor-related orphan nuclear receptor gamma, Th17: T helper 17, IL: interleukin, TNF: tumor necrosis factor. ^*p<0.05, ^**p<0.01.