REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat polyclonal anti-PbUIS4	Sicgen	Cat#AB0042; RRID: AB_2333158
Mouse monoclonal anti-Flag® (M2 clone)	Sigma-Aldrich	Cat#F3165; RRID: AB_259529
Mouse monoclonal anti-V5	Invitrogen	Cat# R960-25; RRID: AB_2556564
Rat monoclonal anti-mCherry, clone 16D7	Invitrogen	Cat#M11217; RRID: AB_2536611
Rat monoclonal anti-mCD81, clone MT81	Silvie et al., 200649	N/A
Rat monoclonal anti-Plasmodium AMA1, clone 28G2	BEI Resources	MRA-897A
Rabbit polyclonal anti-PbTRAP	Klug et al., 202050	N/A
Goat polyclonal anti-GFP	Sicgen	Cat# AB0066-200; RRID: AB_2333101
Rabbit polyclonal anti-GFP	Proteintech	Cat#50430-2-AP; RRID: AB_11042881
Goat anti-mouse IgG AF594	Molecular Probes	Cat#A-11032; RRID: AB_2534091
Donkey anti-rabbit IgG AF594	Molecular Probes	Cat#A-21207; RRID: AB_141637
Goat anti-rat IgG AF594	Molecular Probes	Cat# A-11007; RRID: AB_141374
Goat anti-mouse atto647N	Antibodies-online GmbH	Cat#ABIN964964
Goat anti-mouse STAR RED	Abberior	Cat#STRED-1001
Goat anti-mouse AF680	Thermo Fisher Scientific	Cat#A21058; RRID: AB_2535724
Donkey anti-goat IgG AF680	Thermo Fisher Scientific	Cat#A-21084; RRID: AB_141494
Goat anti-rabbit IgG AF680	Thermo Fisher Scientific	Cat#A-21109; RRID: AB_2535758
Goat anti-mouse IgG DyLight™ 800	Thermo Fisher Scientific	Cat#SA5-3552; RRID: AB_2556774
Bacterial and virus strains
XL1-Blue Competent Cells	Agilent	Cat#200249
Chemicals, peptides, and recombinant proteins
Lipofectamine 2000 reagent	Life Technologies	Cat#11668019; CAS: 158571-62-1
Dextran Tetramethylrhodamine	Invitrogen	Cat#D1817
ProLong™ Diamond Antifade Mountant	Life Technologies	Cat#P36970; RRID: SCR_015961
RFP-Trap Agarose beads	Chromotek	Cat#rta-20
Pyrimethamine	MP Biologicals	Cat# 0219418025 CAS: 58-14-0
Formaldehyde	Electron Microscopy Sciences	Cat#15714 CAS: 50-00-0
Triton X-100	Sigma-Aldrich	Cat#T8787 CAS: 9036-19-5
Hoechst 33342	Invitrogen	Cat#H3570 CAS: 23491-52-3
Igepal® CA-630	Sigma-Aldrich	Cat#56741 CAS: 9002-93-1
Protein G Sepharose™ 4 Fast Flow	Sigma-Aldrich	Cat#17-0618-02
Anti-FLAG®M2 Affinity Gel	Sigma-Aldrich	Cat#A2220
Critical commercial assays
In-Fusion HD Cloning Kit	Clontech	Cat#638911
DNeasy Blood & Tissue Kit	Qiagen	Cat#69504
Plasmid Maxi Kit	Qiagen	Cat#12162
Human T Cell Nucleofector™ Kit	Lonza	Cat#VPA-1002
Experimental models: Cell lines
Mouse: Hepa1-6 hepatic cells	ATCC	ATCC: CRL-1830; RRID: CVCL_0327
Human: HepG2 hepatic cells	ATCC	ATCC: HB-8065; RRID: CVCL_0027
Human: HepG2/CD81 hepatic cells	Silvie et al., 200649	N/A
Experimental models: Organisms/strains
Mouse: C57BL/6JRj	Janvier Labs	N/A
Mouse: BALB/cJRj	Janvier Labs	N/A
Mouse: RjOrl:SWISS (CD-1)	Janvier Labs	N/A
Anopheles stephensi mosquitoes	Radboud University Medical Center	N/A
Plasmodium berghei ANKA strain, clone 15cy1	BEI Resources	MRA-871
Plasmodium yoelii 17XNL strain, clone 1.1	BEI Resources	MRA-593
Plasmodium berghei ANKA PbGFP	Manzoni et al., 201425	N/A
Plasmodium yoelii 17XNL PyGFP	Manzoni et al., 201425	N/A
Plasmodium berghei ANKA PbΔp12p	This study	N/A
Plasmodium berghei ANKA PbΔp38	This study	N/A
Plasmodium berghei ANKA PbΔb9	This study	N/A
Plasmodium berghei ANKA PbΔp36	Manzoni et al., 20176	N/A
Plasmodium yoelii 17XNL PyΔp12p	This study	N/A
Plasmodium yoelii 17XNL PyΔp38	This study	N/A
Plasmodium yoelii 17XNL PyΔb9	This study	N/A
Plasmodium yoelii 17XNL PyΔp36	This study	N/A
Plasmodium berghei ANKA PbGFP::RON4mCherry	This study	N/A
Plasmodium berghei ANKA PbΔb9::RON4mCherry	This study	N/A
Plasmodium berghei ANKA PbΔp36::RON4mCherry	This study	N/A
Plasmodium berghei ANKA PbB9-Flag	This study	N/A
Plasmodium berghei ANKA P36-mCherry	This study	N/A
Plasmodium berghei ANKA PbΔb9 complemented PbB9	This study	N/A
Plasmodium berghei ANKA PbΔb9 complemented Δprop	This study	N/A
Plasmodium berghei ANKA PbΔb9 complemented Δprop6cys1	This study	N/A
Plasmodium berghei ANKA PbΔb9 complemented Pyprop	This study	N/A
Plasmodium berghei ANKA PbΔb9 complemented Pyprop6cys1	This study	N/A
Plasmodium berghei ANKA PbΔb9 complemented Pfprop	This study	N/A
Plasmodium berghei ANKA PbΔb9 complemented Δprop-Flag	This study	N/A
Plasmodium berghei ANKA PbΔb9 complemented Pfprop-Flag	This study	N/A
Oligonucleotides
Primers for plasmids constructs and parasite genotyping, see Table S4	This paper	N/A
Recombinant DNA
GOMO-GFP plasmid	Manzoni et al., 201425	Addgene Plasmid #60975
B3D + mCherry plasmid	Silvie et al., 200851	N/A
pCEN-SPECT2 plasmid	Iwanaga et al., 201052	N/A
pEF1α-AcGFP1-N1 plasmid	Clontech	Cat# 631973
Software and algorithms
ImageJ	Schneider et al., 201253	https://ImageJ.nih.gov/ij/
PSIPRED 4.0	Buchan and Jones, 201954	http://bioinf.cs.ucl.ac.uk/psipred/
Inter-Pro	Mulder et al., 200255	https://www.ebi.ac.uk/interpro/
HHpred	Söding et al., 200556	https://toolkit.tuebingen.mpg.de/tools/hhpred
NetGPI tool	Gíslason et al., 202157	https://services.healthtech.dtu.dk/service.php?NetGPI
IUPred2A web server	Mészáros et al., 201858	https://iupred2a.elte.hu/
Robetta web server	The Baker lab	https://robetta.bakerlab.org/
GalaxyRefine	Heo et al., 201359	https://bio.tools/galaxyrefine
Yasara	Krieger et al., 200960	http://www.yasara.org/
MolProbity	Chen et al., 201061	http://molprobity.manchester.ac.uk/
Prosa II	Wiederstein and Sippl, 200762	https://prosa.services.came.sbg.ac.at/prosa.php
UCSF Chimera	Pettersen et al., 200463	https://www.cgl.ucsf.edu/chimera/
Adaptive Poisson-Boltzmann Solver	Baker et al., 200164	https://mccammon.ucsd.edu/iapbs/
PDB2PQR v.2.1.1	Dolinsky et al., 200465	https://www.cgl.ucsf.edu/chimera/docs/ContributedSoftware/apbs/pdb2pqr.html
MAFFT version 7	Katoh and Standley, 201366	https://mafft.cbrc.jp/alignment/server/
PhyML v3.0	Guindon et al., 201067	http://www.atgc-montpellier.fr/phyml/
Smart Model Selection (SMS)	Lefort et al., 201768	http://www.atgc-montpellier.fr/sms/
aLRT SH-like method	Anisimova et al., 200169	http://www.atgc-montpellier.fr/phyml/alrt/
GP4Rate tool: FuncPatch (now offline)	Huang and Golding, 201470	http://info.mcmaster.ca/yifei/FuncPatch/
X!Tandem pipeline (version 0.2.36)	Langella et al., 201771	https://www.thegpm.org/TANDEM/
R version 3.5.2	R Foundation for Statistical Computing	https://www.r-project.org/
GraphPad Prism 7	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Huygens Professional Deconvolution software v18.10	Scientific Volume Imaging	https://svi.nl/Huygens-Essential