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. 2023 Feb 6;6(4):e202101186. doi: 10.26508/lsa.202101186

Figure 5. RIF1 is required for full recruitment of cohesin subunits at stalled forks.

Figure 5.

(A) Scheme explaining the principle of proximity ligation assay (PLA) between EdU and replisome components. (B) Immunofluorescence analysis of PLA signal between EdU and SMC1 and between EdU and PCNA upon 30-min treatment with 0.1 μM APH in HeLa S3 cells expressing shRNAs against luciferase or RIF1. EdU-positive cells were labeled with Alexa Fluor 555. (C) Level of PLA signal within the nucleus was quantified using CellProfiler. Graphic representation of the PLA signal; at least 100 cells were quantified in each condition. For statistical analysis, a Mann–Whitney test was used, ns, non-significant; ****P < 0.0001; and ***P < 0.001.