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. 2023 Feb 6;6(4):e202101186. doi: 10.26508/lsa.202101186

Figure S3. Treatment of RIF1 depleted-cells with aphidicolin does not perturb DNA synthesis or replisome stability.

Figure S3.

(A) Repetition of DNA fiber experiment from Fig 3F. HeLa S3 cells were labeled for 30 min with IdU and then for 30 min with CldU in the absence or presence of 0.05 μM aphidicolin (APH) in the cell culture medium. Graphic representation of the ratios of CldU versus IdU tract length. For statistical analysis, a Mann–Whitney test was used, ****P < 0.0001. The horizontal bar represents the median with the value indicated in red. At least 50 replication tracts were measured for each experimental condition. (B) Western blot analysis of indicated proteins after immunoprecipitation with an antibody directed against PCNA or against mouse IgG. When indicated, HeLa S3 cells (shLUC or shRIF1) were treated for 30 min with 0.1 μM aphidicolin (APH).