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(A) Flow cytometry analysis of asynchronous HeLa S3 cells blocked for 18 h with thymidine (T0), then released for 2 (T2), 4 (T4), and 8 (T8) hours. Total DNA was stained using propidium iodide and newly synthesized DNA by EdU labeled with Alexa Fluor 488 using click chemistry. (B) Scheme explaining how replication organization is impacting iPOND efficiency.
