Figure 1.

MiR-181a-5p decreases cell viability and survival of UM cells. A. OMM2.5 and UM001 cells were transfected with 25 nM of miR-181a-5p or scrambled miRNA negative control (miR-NC) for 48 h. Cell viability was measured with PrestoBlue^TM assays, and values are presented as a percentage of control (CTL). *P < 0.05, ***P < 0.001 vs. miR-NC. B. Cell proliferation of miR-181a-5p transfected OMM2.5 cells, assessed according to [³H]-thymidine DNA incorporation. Relative proliferation rates are presented as a percentage of CTL. ***P < 0.001 vs. miR-NC. C. Representative figures of flow cytometry results (left) of OMM2.5 cells stained with PI and Annexin V after treatment with miR-181a or miR-NC. The percentage of apoptotic cells is quantified (right). ***P < 0.001 vs. miR-NC. D. After treatment with miR-181a-5p for 48 h, OMM2.5 cells were seeded into 6-well plates and incubated for 21 days. Representative images of colony formation of OMM2.5 cells treated with miR-181a-5p or miR-NC for 48h (left). Colonies were counted after 21 days (shown as percentage of CTL) from 3 independent experiments in triplicate (right). Values are means ± standard error of the mean. ***P < 0.001 vs. miR-NC.