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. 2022 Dec 21;15(2):e16796. doi: 10.15252/emmm.202216796

Figure 3. Expression of IL‐4 in eosinophils depends on their localization.

Figure 3

  • A, B
    Identification of eosinophil populations using SPADE analysis 4 (panel A) and 24 h (panel B) after zymosan injection.
  • C, D
    Representative MELC images showing the IL‐4 and Siglec F expression 4 h (panel C) and 24 h (panel D) after zymosan injection. The white dotted lines depict the area where the transition between the neighboring regions occurs. Size bar represents 100 μm.
  • E
    Number of IL‐4‐expressing eosinophils in the core region and the surrounding area 4 h after zymosan injection. Data are shown as mean (n = 5 mice) ± SEM, two‐tailed Students t‐test ***P < 0.001.
  • F
    Number of IL‐4‐expressing eosinophils in the three regions 24 h after zymosan injection. Data are shown as mean (n = 5 mice) ± SEM, one‐way ANOVA/Bonferroni **P < 0.01, ***P < 0.001.
  • G
    FACS analysis of the number of eosinophils at the indicated time points after zymosan injection in the inflamed paw. Data are shown as mean (n = 5 mice) ± SEM, one‐way ANOVA/Bonferroni *P < 0.05, **P < 0.01, ***P < 0.001.

Source data are available online for this figure.