Figure 7. Eosinophil depletion increases neutrophil recruitment and lowers efferocytosis.

• A
  Concentrations of cytokines and chemokines were determined by multiplex cytokine assay 8 and 24 h after injection of zymosan in paws from control or eosinophil‐depleted mice. Data are mean ± SEM (n = 6 mice). Student's one‐way ANOVA, significance between control and anti‐Siglec F antibody treatment *P < 0.05; **P < 0.01; ***P < 0.001.
• B, C
  FACS analysis of the neutrophil count in paws (panel B) and peripheral blood (panel C) from control or eosinophil‐depleted mice with or without IL‐4c treatment. Data are shown as the mean ± SEM (n = 6–8). Two‐way ANOVA/Bonferroni, **P < 0.01, ***P < 0.001.
• D
  Same as panel B except that efferocytosis was determined. *P < 0.05; ***P < 0.001.
• E
  FACS analysis of alive and dying neutrophils in paws from control or eosinophil‐depleted mice. Data are shown as the mean ± SEM (n = 5 mice).

Source data are available online for this figure.