Figure EV3. Design and validation of plasmid used for testing therapeutic potentials.

1. Illustration of plasmid construct used for therapeutic treatment.
2. Representative images of plasmids tested in HEK cells.
3. Representative confocal images of long propriospinal neuron retrogradely labeled with FluoroGold (blue), FGF22 overexpression (green) and CST collateral (white) contacting FGF22 overexpressing neuron.

Data information: Scale bar in (B) equals ∼ 30 μm and (C) equals 5 μm.