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. 2023 Jan 5;15(2):e16111. doi: 10.15252/emmm.202216111

Figure EV4. Chemogenetic silencing of the hindlimb motor cortex demonstrates its contribution to functional recovery following spinal cord injury.

Figure EV4

  1. Time line of the experiment in which FGF22 is overexpressed in long propriospinal neurons and silencing DREADDs are delivered to the hindlimb motor cortex.
  2. Confocal images of the DREADDs expressing neurons retrogradely labeled with AAV‐Cre (Neurotrace: blue; Retrogradely labeled neurons: green; DREADD+ neurons: red). Scale bar equals 100 μm.
  3. Longitudinal quantifications of mistakes in the irregular ladder rung at baseline, 3‐day postinjury (dpi), 14 dpi, 28 dpi before CNO administration (green dots), 28 dpi 30 min after CNO administration (red dots) and 29 dpi (CNO washout; gray dots) and scheme of the ladder rung task.

Data information. ns: P > 0.05; *P < 0.05 and **P < 0.01. For general group difference one‐way repeated‐measures ANOVA was used. In order to assess recovery of individual mice paired t‐test was used (panel C).