Figure 2. Proximal tubular‐specific deletion of Lonp1 aggravates renal injury and mitochondrial dysfunction in UUO model.

• A
  Deposition of total fibrosis in kidney tissues was determined by Masson's trichrome staining. Scale bar: 50 μm.
• B
  Sirius red staining in WT and cKO mice after UUO. Scale bar: 50 μm.
• C
  Fibrotic area statistics of Sirius red staining in WT and cKO mice after UUO. N = 5 in Sham groups. N = 7 in UUO groups, biological replicates.
• D, E
  Western blot and densitometric analysis for the expression of FN1, Collagen I, Collagen III and α‐SMA in WT and cKO mice after UUO (n = 3, biological replicates).
• F
  qRT‐PCR analysis of FN1, Collagen I, Collagen III and α‐SMA in WT and cKO mice after UUO (n = 7 or 5 in Sham groups, n = 9 in UUO groups, biological replicates).
• G
  qRT‐PCR analysis of mtDNA expression in WT and cKO mice after UUO (n = 7 or 5 in Sham groups, n = 7 in UUO groups, biological replicates).
• H
  Mitochondrial respiratory chain complex I enzymatic activity in WT and cKO mice after UUO (n = 4, biological replicates).
• I
  Transmission electron microscopy images of the mitochondria in tubular cells in WT and cKO mice after UUO. Scale bar: 500 nm.

Data information: Data are presented as mean ± SEM. One‐way ANOVA.

Source data are available online for this figure.