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. 2023 Feb 8;16:58. doi: 10.1186/s13071-023-05680-w

Table 6.

Characteristics and main limitations of different copromicroscopic techniques used for the diagnosis of helminth infection in ruminants

FEC technique Diagnostic performance Technical performance Comment
Detection limit Accuracy Precision Cost Processing timeb Equipment needs
McMaster Mediuma Low Low Inexpensive Medium Basic laboratory equipment Most common technique across the globe
Modified Improved McMaster Medium Low Low Low Medium Fully equipped laboratory
Modified Wisconsin Very Low Low Very low Low Long Fully equipped laboratory Lack of precision due to the lack of a grid on the coverslip
Mini-FLOTAC Low High High Low Very long Basic laboratory equipment Allows detection of GIN, lungworm larvae and trematodes
FLOTAC Very Low Very high Very high Low Very long Fully equipped laboratory Requires centrifugation steps with two different rotors
FECPAK Medium Low Low Expensive Long All equipment is provided by the manufacturer It is a pen-sided tool. Utility is limited to gastrointestinal strongyles

GIN Gastrointestinal nematodes

aDetection limit of McMaster. The efficacy of the modified McMaster can be increased by changing the fecal/fluid ratio and/or reading several chambers

bMedium: 6 min/sample; Long: 13 min/sample [37]