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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2022 Dec 14;194:284–297. doi: 10.1016/j.freeradbiomed.2022.12.013

Fig. 5.

Fig. 5.

Zebrafish were treated with 7 μM DMF during protruding-mouth stage for 6 hours and then immediately fixed and Nrf2a protein was labeled via Immunohistochemistry (IHC). Images of the liver were taken using a confocal microscope under a 40x objective. A) Liver mean fluorescence intensity (FI) of Nrf2a protein was determined via image analysis. B) Representative images of the zebrafish liver are shown where the liver is circled in yellow, TRITC (red) represents Nrf2a, and DAPI (blue) represents nuclei. Calculations were performed using a one-way ANOVA followed by Fisher’s LSD post-hoc test. N = 8–13 fish. Different letters indicate significant differences (p ≤ 0.05) between treatment and genotype.