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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2022 Dec 14;194:284–297. doi: 10.1016/j.freeradbiomed.2022.12.013

Fig. 7.

Fig. 7.

A) Zebrafish were treated with 7 μM DMF during the pharyngula, hatching, and protruding-mouth stage for 6 hours, and then embryos were treated with BioGee for 2 hrs before fixation 24 hours after treatment, and BioGee protein conjugates were labeled in situ. B) Mean fluorescence intensity (FI) of BioGee protein conjugates of the body tissue was determined via image analysis. Representative heatmaps generated to visualize BioGee-protein conjugate florescence at the C) pharyngula/hatching, D) hatching/protruding-mouth, and E) hatching/protruding-mouth stage are shown. Calculations were performed using a two-way ANOVA followed by Fisher’s LSD post-hoc test. N = 8–10 fish. Different letters indicate significant differences (p ≤ 0.05) between treatment, genotype, and time point.