Fig. 6. The immunogenic effect of the treatment of B16F10-bearing mice. Evaluation of the immunogenic effect of the treatment of B16F10-bearing mice by flow cytometry upon intratumor injection of Ir-pbt-Bpa (3 mg kg⁻¹) into the primary tumor and exposure to two-photon irradiation (750 nm, 50 mW, 5 min). (a) Determination of the CD8/Foxp3 ratio in the tumors. (b) Percentages of matured dendritic cells in the lymph nodes on day 7. (c–h) Percentages of CD8⁺ T cells, effector memory T cells (T_EM), and regulatory T cells (T_reg) in the primary tumor or the spleen. Mean ± standard deviation (n = 3). (1) Control; (2) light only; (3) Ir-pbt-Bpa only; (4) Ir-pbt-Bpa + light. ***P < 0.001, **P < 0.01 and *P < 0.05, in comparison to control.