Figure 1.

Three illustrations of protein interactions and their descriptions by machine readable text of the sort that would automate database entry. (A) A set of four proteins (A, B, C and D) have been studied and shown to interact by a given method. The condensed text is shown below. (B) Example of the SWI6–SWI4 interactions observed by Siegmund and Nasmyth (15) using immunoprecipitation, copurification and in vitro binding. In this case the protein PIR codes are used RGBYW6 for SWI6p and S50614 for SWI4p. (C) Example of the Zip3 interactions demonstrated by two-hybrid screen and immunoprecipitation by Agarwal and Roeder (16). In this case S55950 (Zip3p) can be immunoprecipitated with RA57p (RA57_YEAST), MR11p (MR11_YEAST) and Zip2p (YGZ9_YEAST). Two-hybrid screen was performed and showed interactions of Zip3p with ZIP1p, MSH5p, RAD57p, RAD51p.