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. Author manuscript; available in PMC: 2023 Feb 8.
Published in final edited form as: Cell. 2023 Feb 2;186(3):621–645.e33. doi: 10.1016/j.cell.2022.12.038

Figure 4 – Production of IFN-γ by the lymphoid cells of IRF1-deficient patients.

Figure 4 –

(A) Induction of IFN-γ secretion in a whole-blood assay, for controls (CTLs), IL-12Rβ1-deficient patients and patients. Bars represent the mean. (B) Intracellular flow cytometry on IFN-γ+ PBMCs after stimulation with IL-12, IL-23, or BCG. Bars represent the mean. Technical duplicates of the same experiment are shown for P1 and P2. (C) UMAP analysis of intracellular T-bet and IFN-γ expression by intracellular spectral flow cytometry across various PBMC subsets. Lymphoid subsets based on surface marker expression (upper panel), and their levels of IFN-γ and T-bet expression (lower panel). (D) Cytokine levels in the supernatant of naïve CD4+ T cells in polarizing conditions.