Fig. 3.

Role of HQNO and pyocyanin on bacterial fitness and spatial distribution in murine wounds. (A) CFU per wound of P. aeruginosa or S. aureus in mono- (open circles) and co-infection (closed circles) with wildtype (black), ΔpqsL (red), or Δphz1/2 (blue). P-values were determined using a Mann–Whitney test. (B) Total biomass determined by confocal microscopy of wounds in mono- (open) or co-infection (closed) with P. aeruginosa WT (black), ΔpqsL(red), or Δphz1/2 (blue) mutants. (C) Percentage that planktonic cells that contribute to the total bacterial biomass in wounds in mono- (open) or co-infection (closed) with S. aureus and P. aeruginosa WT (black), ΔpqsL(red), or Δphz1/2 (blue) mutants. (D) The enrichment distance of P. aeruginosa from S. aureus in microns. Measurements from images taken from the wound core are shown with circles, while images taken from the wound edge are shown with triangles. P-value was determined by a Student’s t test. (E) Relationship between bacterial density and enrichment distance in the core (circles) or edge (triangles) of murine wounds coinfected with S. aureus and P. aeruginosa WT (black), ΔpqsL(red), or Δphz1/2 (blue).