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. 2022 Dec 12;119(51):e2214880119. doi: 10.1073/pnas.2214880119

Fig. 5.

Fig. 5.

FETUA-3 inhibits all three classes of C. atrox SVMPs activities on fibrinogen. Inhibition of fibrinogenolytic activity of MDC-4, MAD-3, and MPO-1 by the five FETUA proteins was tested using bovine fibrinogen as the substrate. Effects of the FETUA inhibitors on SVMP fibrinogenolysis were assessed by observing cleavage of the α and β chains of bovine fibrinogen as decreased band intensity, the positions of the α-, β-, and γ-uncleaved forms are denoted in the control lane in the panel. (A–E) FETUA samples incubated with MDC-4, MAD-3, or MPO1, and fibrinogen, and analyzed by SDS-PAGE. Lane 1—fibrinogen alone lane 2—fibrinogen incubated with SVMP; and lanes 3–5—fibrinogen incubated with increasing amounts of FETUA (1, 2.5, and 5 μg). FETUA inhibition of SVMP fibrinogenolytic activity is indicated by asterisk (*). (F) A summary of FETUA inhibition of MDC-4, MAD-3, and MPO-1. FETUA-3 inhibits all three classes of SVMP. FETUA-2 and FETUA-5 show moderate inhibition of MDC-4 activity (β-chain cleavage) while FETUA-2, FETUA-4, and FETUA-5 inhibit cleavage of α and β chains by MAD-3. MPO-1 activity on fibrinogen is only neutralized by FETUA-3.