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. 2022 Dec 12;119(51):e2214880119. doi: 10.1073/pnas.2214880119

Fig. 6.

Fig. 6.

FETUA-3 binds approximately 21 SVMPs from all three classes. Affinity purification and identification of FETUA-3 interacting venom proteins from C. atrox. (A) SDS-PAGE of C. atrox venom purified on a FETUA-3 affinity column. Lane 1—protein standards, lane 2—whole-venom input, lane 3—flowthrough venom fraction from FETUA-3 affinity column, and lane 4—eluate. Asterisks (*) denote enriched proteins in eluate fraction that migrate at the expected sizes of class III and class I SVMPs. (B) Mass spectrometry of the FETUA-3 column eluate identified 21 SVMPs based on unique peptide spectra (SI Appendix, Supplementary Data 3). All three classes of SVMPs are represented in the eluate fraction.