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. 2022 Dec 12;119(51):e2214335119. doi: 10.1073/pnas.2214335119

Fig. 2.

Fig. 2.

ISG20, TREX1, and ERI1 generate tyRNAs. (A) In vitro guide trimming by 3′→5′ exonucleases at 2 mM MnCl2. Left: a representative gel image for each AGO. Right: the relative amount of each guide length after incubation with either 3′→5′ exonuclease. Mean ± SD. (B) In vitro trimming of AGO2-associated miR-20a by the catalytically dead exonuclease mutants at 2 mM MnCl2. (C) In vitro trimming of AGO2-associated miR-20a by 3′→5′ exonucleases at 2 mM MgCl2. (D) In vitro trimming of different miRNAs by ISG20. Left: a representative gel image. Right: the relative amount of each guide length after ISG20 incubation. Mean ± SD. (E) Docking model of ISG20 (blue) on a guide (red)-bound AGO3 (surface model). (F) In vitro trimming of AGO3-associated different miRNAs, followed by target cleavage.