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. 2023 Feb 8;299(3):102990. doi: 10.1016/j.jbc.2023.102990

Figure 3.

Figure 3

Mproimpairs ISG induction by cleaving HDACs.A, HEK-293T cells were transfected with pCDNA3.1-HDAC2-Flag plasmids together with or without Mpro for 24 h before Western blotting analysis with the anti-flag antibody. B, HEK-293T cells were transfected with HDAC2 expression construct alone or in the presence of increasing concentrations of the Mpro, and after 30 h transfection, cells were lysed for Western blotting analysis. C, HEK-293T cells were transfected with HDAC2 expression plasmid along with Mpro or its mutants Mpro-H41A, Mpro-C145A. Western blotting was performed after 24 h transfection. D, Schematic representation of Class I HDAC with different similarities to the HDAC1. E, HEK-293T cells were transfected with HDAC1, HDAC3, or HDAC8 separately along with either Mpro or empty vector. Western blotting was performed after 24 h transfection. F, HEK-293T cells were transfected with Mpro or empty vector. Cells were then lysed for immunoprecipitation analysis at 32 h after transfection. HDAC, histone deacetylase; ISG, interferon-stimulated gene; Mpro, main protease.