Fig 1. Development of U-251 MG human glioblastoma astrocytoma 3D in vitro cell culture model.

I). Image of U-251 MG 2D cells in T75 flask II) 3D tumoursphere constructed in low adhesion plate. III). 3D tumoursphere constructed in hanging drop plate. IV) 3D tumoursphere constructed in hydroxipropylcellulose scaffold. Tumour spheroid formation was visually confirmed using an Optika XDS-2 trinocular inverse microscope equipped with a Camera ISH500.