| Cell line (Homo sapiens) |
293T |
ATCC |
CRL-3216 |
Periodically checked for mycoplasma and retrovirus contamination not authenticated since purchased directly from ATCC |
| Transfected construct (HIV-1) |
pNL4-3 and CA mutants introduced in pNL4-3 |
This paper |
|
For viral production assay and RT-qPCR gRNA quantification assay |
| Transfected construct (HIV-1) |
NL4-3 (MA-3xHA/PR-) and its derivatives |
This paper |
|
For CLIP experiments and BMH crosslink experiments |
| Antibody |
Mouse monoclonal anti-HIV-1 p24CA |
NIH AIDS Reagent Program |
183-H12-5C |
WB (1:100) |
| Antibody |
Rabbit polyclonal anti-HIV-1 Nucleocapsid |
This paper |
|
WB (1:2000) |
| Commercial assay or kit |
Power SYBR Green RNA-to-CT 1-Step Kit |
ThermoFisher |
Cat# 4389986 |
|
| Chemical compound and drug |
BMH (bismaleimidohexane) |
ThermoFisher |
Cat# 22330 |
|
| Software and algorithm |
Prism |
Graphpad |
|
For CLIP data analysis graphing |
| Software and algorithm |
Image Studio |
LI-COR Biosciences |
|
For western blot band quantification |
| Software and algorithm |
Cutadapt |
https://doi.org/10.14806/ej.17.1.200
|
|
For CLIP data processing |
| Software and algorithm |
FASTX toolkit |
http://hannonlab.cshl.edu/fastx_toolkit
|
|
For CLIP data processing |
| Software and algorithm |
Bowtie |
https://doi.org/10.1186/gb-2009-10-3-r25
|
|
For CLIP data processing |
| Software and algorithm |
SAMTools |
https://doi.org/10.1093/bioinformatics/btp352
|
|
For CLIP data processing |
