Author response image 1. The IHC potential and spontaneous release does not change significantly in the presence of 20 mM TEA and 1mM Cs.

(A) The IHC potential was measured using perforated patch-clamp recordings in current clamp mode. Two different solutions, without and with 20 mM TEA and 1 mM CsCl, were quickly perfused (>100 mL/h) during the recordings. (Ai) The IHC potential was monitored during continuous recordings of 20 s. (Aii) There was no significant change in the IHC potential with the two solutions. The presence of TEA and Cs slightly hyperpolarized the IHC potential by 5.56 ± 1.615 mV (4 cells; p-value = 0.1250, Wilcoxon matched-pairs signed rank test). (B) Spontaneous EPSCs were recorded from individual afferent boutons using continuous ruptured patch-clamp recordings of 20 s. (Bi) We quantified the number of EPSCs occurring during one minute before the start of perfusion and during one minute after the bath solution has been exchanged.