Skip to main content
. 2023 Feb 8;13:2279. doi: 10.1038/s41598-023-29396-0

Figure 3.

Figure 3

Demonstration of storability of the ClO2· oxidized graphene grid. CryoEM image analyses of GroEL was carried out on the EG-grid produced by 5-min epichlorohydrin treatment of a ClO2· oxidized graphene grid stored under N2 gas at room temperature for 3 months. (a) Typical cryoEM image of GroEL on the EG-grid. (b) Selected 2D class averages aligned in descending order of particle numbers from left to right and top to bottom. The classes selected for the following analysis are indicated with red boxes. Right panel shows the angular distribution of particles used in the final refinement. The final 3D map is shown for reference. The efficiency (Eod) calculated with cryoEF is also shown. (c) Two orthogonal views of the final 3D map: top view, left panel; and side view, right panel. The local resolution distribution is colored as in the color bar. (d) The FSC curve for the final map. The dashed blue line indicates the FSC = 0.143 criterion.