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. 2023 Feb 8;13:2279. doi: 10.1038/s41598-023-29396-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CryoEM image analyses of SARS-CoV-2 spike protein on the EG-grid and Quantifoil grid. (a–c) Typical cryoEM images of spike protein on the EG-grid (spike-EG) (a), on the Quantifoil grid (spike-Q) (b), and on the glow discharged graphene grid (spike-G) (c). The protein concentration and the value of electron beam transparency of each image (measured from the image brightness ratio between the energy filter slit in and out) are also shown. (d and e) Selected 2D class averages from the spike-EG dataset (d) and the spike-Q dataset (e). The class averages are aligned in descending order of particle numbers from left to right and top to bottom. (f and g) Angular distributions of particles used in the final refinement for the spike-EG dataset (f) and the spike-Q dataset (g). Final 3D maps are shown for reference. The efficiencies (E_od) calculated with cryoEF are also shown. (h) Two orthogonal views of the final 3D map from the spike-EG dataset: top view of the trimer, left panel; and side view, right panel. The local resolution distribution is colored as in the color bar. (i) The FSC curves for the final maps of the spike-EG and spike-Q dataset. The dashed blue line indicates the FSC = 0.143 criterion.