Figure 2.

C3KO mice do not exhibit increased apoptosis of spinal interneurons. A, Cryosections of P0 spinal cords from control and C3KO pups immunostained for the indicated markers (green) and counterstained with DAPI for nuclei (blue). Scale bar: 200 µm. No gross abnormalities of the spinal cord (previously observed in complete Pcdhg null mutants; Wang et al., 2002b; Prasad et al., 2008) were observed in C3KO mutants, nor were any alterations in the number of FoxP2-labeled ventral interneurons (B), CC3-labeled apoptotic cells (C), or Pax2-labeled dorsal and ventral interneurons (D). ns = not significant.