Figure 9.

The intracellular domain of γC3 is required for Axin1-mediated dendritic arborization. A, B, Quantification of area under the curve for Sholl analysis of control and C3KO cultured cortical neurons transfected with GFP, either Axin1 shRNA or the scrambled control shRNA, and a γC3 construct lacking either the cytoplasmic (C3Δcyto; A), or the extracellular domain (C3Δecto) and analyzed at DIV8. B, Bars to the left indicate reduced complexity; bars to the right indicate increased complexity. C, Schematic depiction of γC3 deletion transfection constructs transfected. D, Representative traces of neurons from each transfection condition. Scale bar: 50 µm. n ≥ 25 neurons per condition. One-way ANOVA; Dunnett's multiple comparisons test. Error bars represent the SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns = not significant.