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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Nature. 2022 Sep 7;610(7933):737–743. doi: 10.1038/s41586-022-05089-y

Figure 1. Distinct requirements for antigen presentation and CCR7 expression in differentiation of iTreg versus pathogenic Th17 cells.

Figure 1.

a-b, Hh-specific T cell proliferation and differentiation in Hh-colonized CD11c-Cre;I-Abf/f (MHCIIΔCD11c) (n=3) and I-Abf/f or CD11c-Cre;I-Abf/+ littermate control mice (n=6) (a) and in Ccr7ΔCD11c (n = 7) and littermate control mice (n = 9) (b). CFSE-labeled naïve TCR transgenic Hh7–2 T cells from the C1 MLN were assessed for cell proliferation and expression of FoxP3 and RORγt at 3 days after adoptive transfer. Representative flow cytometry (left) and aggregate results (right). Data summarize two (a) and three (b) independent experiments. c, Representative H&E histology in large intestine of mice with indicated genotypes. Scale bars are 1 mm and 50μm, for 1.25X and 20X. respectively. All statistics were calculated by unpaired two-sided Welch’s t-test. Error bars denote mean ± s.d. p-values are indicated in the figure.