Figure 8. Iron metabolism analyses under the application of Stattic.

Serum hepcidin (A), Fe (B), ferritin (C), transferrin (D) concentrations; mRNA expression levels of iron metabolism genes hepcidin (E), FTH (F), FTL (G,) and FPN (H) in livers and Western blot analysis of liver iron metabolism markers (I left) and quantification of band intensities (I right) in the control group, in the Stattic group, in the ligature group and those in the combined of ligature and Stattic application group (n=10 in each group). Data are presented as mean ± SD. Between-group comparisons were performed using ANOVA test; *P<0.05, **P<0.01, ***P<0.001 as compared with the control group; ^#P<0.05, ^##P<0.01, ^###P<0.001 as compared with the ligature group.