Figure 7.

Altered distribution of V_H segments in the IgD-negative mature naïve B cell population Distribution of different V_H (A) and J_H (B) segments used in CD19⁺CD27^-CD21⁺CD10^-IgM⁺IgD^- mature naïve B cells is shown as relative difference compared to the matched IgD⁺ counterpart. Symbols represent the Δ frequency IgD^-/mutant versus IgD⁺/wild-type from individual samples and bars the mean ± SEM. V_H and J_H segments are ordered on the x-axis according to their mean relative difference (*, p < 0.05; **, p < 0.01; one sample t-test with hypothesized mean of 0). (C) Representation of species richness (q=0), Shannon diversity index (q=1) and Simpson diversity index (q=2) is shown as difference between IgD^- and IgD⁺ B cell subsets. Symbols represent the Δ value IgD^-/mutant versus IgD⁺/wild-type of each parameter from individuals samples and bars the mean ± SEM. (D) Distribution of CDR3 IgH length in IgD⁺ (blue) and IgD^- (red) mature naïve B cells. Each symbol represents the mean frequency ± SEM of a distinct CDR3 length.