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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: J Autoimmun. 2022 Dec 2;134:102961. doi: 10.1016/j.jaut.2022.102961

Figure 2 |. CAR-transduced Tregs maintain their phenotype and suppressive function.

Figure 2 |

(A) Timeline for generating human CAR Tregs. (B) CAR and ΔNGFR expression before enrichment of ΔNGFR+ cells on day 7. (C) FOXP3 and Helios expression on day 8. (D) CpG methylation in the TSDR as determined by pyrosequencing on day 8. (E) Suppression of polyclonally-stimulated Tresp proliferation by CAR Tregs in the absence of CAR target antigens. Data in (B), (C) and (E) were acquired by flow cytometry, gated on live CD4+ cells. Averaged data are mean ± SEM with each connected series representing one individual. n=6–17 with data collected in 2–6 individual experiments. Statistical significance was determined using paired two-tailed Student’s t-tests (C/D) and two-way ANOVA (E) with p-values shown.