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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: J Autoimmun. 2022 Dec 2;134:102961. doi: 10.1016/j.jaut.2022.102961

Figure 3 |. FliC-CAR Tregs are activated in the presence of their target antigen.

Figure 3 |

FliC-CAR (red) and HER2-CAR (blue) Tregs were cultured in plates coated with varying concentrations of rFliC and rFla2 (A-C) Activation-induced expression of CD69 (A), PD1 (B), LAP and GARP (C) was measured by flow cytometry after 24 hours, gated on live CD4+ cells. (D) FliC-CAR and HER2-CAR Tregs were activated with soluble fragmented flagella from 042 or HS bacteria and expression of the indicated markers was analyzed after 24 hours. Averaged data are mean + SEM with each connected series representing one individual. n=4–6 with data collected in 2–3 individual experiments. Statistical significance was determined using one-way ANOVA with p-values shown.